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Scientific Details About TNF ELISA

The TNF-α or Tumor Necrosis Factor Alpha ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood. Transcription related growth factors and stimulating factors are complex subunits of proteins involved in cell differentiation.

Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. Enzyme-Linked Immunosorbent Assays, Human proteins and human recombinants are used in quantitate human TNF ELISA kits and to produce anti-human mono and polyclonal antibodies.

Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Tumor necrosis factor is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction.

It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNF ELISA kit allows the detection and quantification of endogenous levels of natural or recombinant Human TNF alpha in plasma, serum, and lysates.

The Human TNF Alpha (TNF-α) ELISA employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-α present is bound by the immobilized antibody. Tumor necrosis factor-alpha is a cytokine involved in systemic inflammation.